Abstract

A CENTRAL COMPOSITE DESIGN APPROACH FOR QUANTIFYING LIPID NANOPARTICLES USING UV-TRANSMITTANCE

Samir Bhargav* and N. V. Satheesh Madhav

Abstract

The present work reported an economic approach for SLN development using a Bath-Sonicator. In our method Transmittance (T%) measures the effects of Dextran, Tween 80, Sonication time and Lipid Concentration over formation of Particles below 300 nm. In our brute-force methodology, the modified Solvent-evaporation method has been used for nanoparticle formation. The variables have been optimised using Central Composite Design, to find best SLN formulation with highest T%. The Particle Size analyser has been used for conforming the effect of variables over T% and for supporting our determinations. The Quadratic model from response surfaces for \T%- 300 nm was found significant. The best SLN preparation is made with 0.5-1% Tween 80, 12 mg Dextran, 8 mg LC and 18 min Sonication time. It has maximum T%-300 nm (83.75%) respectively, with Zeta potential as 23 mV. This proposed method can be used for formulating SLNs, along with the determination of Particle size range. The method is economic, by involving a UV Spectrophotometer based approach. Results reveal that there is no insignificant difference between Particle sizes as determined using Spectrophotometer and Malvern Zetasizer.

Keywords: Solid Lipid Nanoparticles (SLN); Central Composite Design (CCD); Transmittance (T%); Lipid Concentration (LC); Sonication Time (ST).