Abstract

MICROBIAL SYNTHESIS OF SILVER NANOPARTICLES BY USING FISH PATHOGEN AEROMONAS SALMONICIDA

D. Vineela, S. Janardana Reddy* and B. Kiran Kumar

Abstract

Microbial synthesis of silver nanoparticles using bacteria has received profound interest because of their potential to synthesize nanoparticles of various size, shape and morphology. In the present study, synthesis of silver nanoparticles was done by bacterial strain Aeromonas salmonicida. Nanoparticles are used immensely due to its small size, orientation, physical properties, which are reportedly shown to change the performance of any other material which is in contact with these tiny particles. These particles can be prepared easily by different chemical, physical, and biological approaches. But the biological approach is the most emerging approach of preparation, because, this method is easier than the other methods, ecofriendly and less time consuming. The synthesis of silver nanoparticles was successfully observed by the formation of colourless aqueous solution to reddish brown colour after the 48hrs of incubation with bacterial strain Aeromonas salmonicida. Synthesis of silver nanoparticles takes place due to excitation of Surface Plasmon Resonance (SPR) of silver ions. UV-VIS spectroscopy also recorded and the spectra showed a maximum absorption peak at a visible range of 300nm that confirms the formation of silver nanoparticles. Silver nanoparticles shows greatest antibacterial and antifungal activity and also useful in aquatic and biomedical applications.

Keywords: Silver Nanoparticles, Aeromonas salmonicida, Microbial Synthesis, UVVisible Spectrophotometer.