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Abstract

DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR ESTIMATION OF NIMODIPINE CONTENT BY UVSPECTROSCOPIC METHOD

Rajesh S. Jadhav, Milind Ubale and Jagdish V. Bharad*

Abstract

A Simple, specific, rapid, precise and accurate UV Spectrophotometric method have been developed and Validated for determination of Nimodipine formulation drug. Nimodipine showed the absorption maxima in at 239.0 nm and was linear for a range of 5 μg/ml–25 μg/ml with correlation coefficient of 0.9996. The validation of the above proposed method was done by carrying out precision and accuracy studies. The analytical method showed good Intra precision (Repeatability) with relative standard deviation 0.522% and Inter precision with relative standard deviation is 0.355% which is less than 2. The percentage recovery at three different levels i.e. 50%, 100% and 150% was found to be 49.9%, 99.1% and 149.6% respectively. The proposed method was validated for the parameter Specificity, Precision, Linearity and range, Ruggedness, Accuracy and recovery. Hence proposed analytical method for estimation of Nimodipine formulation drug by UV spectrophotometer in pharmaceutical can be applied for the routine quality control analysis.

Keywords: Validation, Nimodipine, UV Spectrophotometer.


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