Abstract

ISOLATION OF BACTERIOCIN PRODUCING LACTIC ACID BACTERIA FROM BUFFALO MILK

Deepali Chittora* and Kanika Sharma

Abstract

The purpose of the study have to establish the antimicrobial activity of bacteriocin producing lactic acid bacteria is isolated from raw milk of buffalo. Fifteen lactic acid bacteria isolates based upon the distinct morphology were isolated from the samples and identified as Lactic acid bacteria according to phenotypic characteristics. Bacteriocin producing organisms were screened by Agar well diffusion assay test. There is 9 isolates were able to produce bacteriocin whose antibacterial activity was analyzed by agar well diffusion assay test against indicator organisms. This indicator bacterium included Bacillus mycoides, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pnuemoniae and Proteus vulgaris were inhibited by the isolates. The bacteriocin producing lactic acid bacteria have probiotic properties like antimicrobial activity, gelatinase activity, blood hemolysis, antibiotic sensitivity and bile salts hydrolases activity (BSH). Lactic acid bacteria from raw milk samples that inhibited certain pathogenic organisms by producing bacteriocin may be beneficial for a probiotic culture to be successful in colonizing and to challenge with pathogens.

Keywords: Bacillus mycoides, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pnuemoniae, Proteus vulgaris, Bacteriocin, Isolates.