Abstract

CHEMOTHERAPEUTIC POTENTIAL OF GRAPE SEED EXTRACT (VITIS VINIFERA) AGAINST CYCLOPHOSPHAMIDE INDUCED OXIDATIVE STRESS IN MICE

Naima Z. Mohamed and Hanan F. Aly

Abstract

Cyclophosphamide (CP) is one of the most potent chemotherapeutic antitumor drugs. Oxidative stress has been proven to be involved in cyclophosphamide-induced toxicity. Damage to normal tissues due to toxic metabolites limits the usage of CP efficiently for treating various cancers. Therefore, the present study was undertaken to examine the antioxidant potential of low and high dose (100 and 300 mg/kg body weight) of grape seed extract (GSE) against the toxicity of cyclophosphamide have been evaluated in adult Swiss albino mice. Cyclophosphamide treated animals revealed significant elevation of liver markere enzymes ; alanine aminotransferase (ALT) aspartate aminotransferase (AST) and alkaline phosphatase (ALP), while the activities of antioxidant enzymes glutathione reductase (GR) and thioredoxin reductase (TrxR) were decreased in liver, colon and testis of mice. Furthermore, mice administered cyclophosphamide showed a marked increase in DNA tail length, percentage of DNA in tail as well as DNA tail moment (comet assay parameters) and GSE significantly attenuated them.

Keywords: Cyclophosphamide, Grape seed extract, Antioxidants, Liver, Colon, Testis.