Abstract

OPTIMIZING THE REACTION CONDITIONS FOR EXTRACELLULAR TANNASE FROM ASPERGILLUS TPF-4

Sunny Dhiman* and Gunjan Mukherjee

Abstract

The present research investigation reports an attempt to optimize the reaction conditions for previously purified tannase from Aspergillus sp. TPF-4. Buffer system, buffer pH, buffer molarity, reaction temperature & reaction time were the main parameters that were optimized for maximum activity of Aspergillus TPF-4 tannase. Furthermore the effect of various metal ions and inhibitors on specific activity of enzyme was also assessed. 0.05M citrate buffer (pH 5.5) was reported as the most prodigious for maximal activity of tannase amongst various buffers tested. The optimum temperature and best reaction time recorded for maximum tannase activity was 45°C & 3 minutes respectively. Sodium azide was reported as the most potent inhibitor of tannase activity amongst various metal ions and inhibitors tested. The prospects of tannin hydrolysis by purified tannase from Aspergillus TPF-4 tannase look quite promising. Thus future studies to enhance the tannin hydrolysis rate as well as to assure enhanced process control for increased activity of tannase would be envisaged.

Keywords: Purified tannase, Aspergillus TPF-4, optimization, potent, inhibitor, maximal activity.