WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

BIOANALYTICAL METHOD VALIDATION FOR PERINDOPRIL AND AMLODIPINE IN HUMAN PLASMA USING UPLC/ESI-MS/MS

Kalaiyarasi D., K. S. Jaganathan* and M. Vamsi Krishna

Abstract

Background: The main objective of this study is to develop and validate a simple mass compatible method for quantification of both perindopril and amlodipine in human plasma. Methods: A UPLC/ESIMS/ MS method for the determination of combined dosage form of perindopril and amlodipine in human plasma sample was developed. The gradient elution with flow rate at 0.3 mL per min of mobile phase was kept and 10 μL of sample was injected in each run. The total chromatographic run time was 5.5 min. Argon was used as the collision gas at the pressure of 3.5X10-5 Torr. Results: In this developed method, a high recovery of perindopril and amlodipine in plasma samples was proved with improved quality data in terms of increased detection limits and chromatographic resolution with greater sensitivity. As per ICH guidelines, the main characteristics of a bioanalytical method validation constraints that are essential to confirm the suitability and reliability of analytical results were evaluated. Conclusion: Quantification of perindopril and amlodipine dosage forms by this method is time saving, cost effective and it can be used in clinical studies from PK studies or clinical trials using LC-MS/MS to quantify the drug content in human plasma samples.

Keywords: Amlodipine, Bioanalytical method, Perindopril, Plasma.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More