Abstract

WOUND HEALING EFFECT OF METHANOLIC EXTRACT OF SYZYGIUM CUMINI (L.) LEAVES AND ITS COMBINATION WITH CLARIFIED BUTTER GHEE IN ALBINO WISTAR RATS

Bhandare Supriya* and Chapole Nilkanth**

Abstract

Syzygium cumini L., commonly known as Jamun/Jambolan, are consumed widely in India. However, little is known about wound healing properties of leaves. Present study was aimed to evaluate effect of methanolic extract of syzygium cumini L. (MESC) leaves and its combination with clarified butter ghee (G) using incision and excision model on albino wistar rats. Acute toxicity studies revealed the nontoxic nature of MESC and previous articles also report the cow ghee is non-toxic. Wistar albino rats (150 − 200 gm) were randomly divided into six groups (n = 6), namely Vehicle control, Framycetin skin cream (STD), MESC 200 mg/kg, MESC 400 mg/kg, MESCG 200 mg/kg (Methanolic extract of syzygium cumini L. and ghee) and MESCG 400mg/kg all doses applied topically once in a day. In incision model treatment were given for 9 days from day of wound and the tensile strength of wounds were estimated. In excision model, treatment given till 16th day of wound and percentage of wound closure and period of epithelization was calculated. Data was analyzed by unpaired t test and p < 0.0001 is considered as significant. Topical application of low and high dose of MESC and MESCG showed the wound healing potential which promoted wound contraction, reduced the wound closure time and increase in tensile strength. Thus, the present study support syzygium cumini L. leaves in the management of wounds and additionally ghee may decrease the form scar of wound.

Keywords: Syzygium cumini, Ghee, Wound closure, Epithelization, Tensile strength, Incision, Excision, MESC, MESCG.