Abstract

OPTIMISATION OF PRESERVATIVE SYSTEM FOR LIQUID DOSAGE FORM USING BOX BEHNKEN DESIGN

Neha Tiwari*, U. V. S. Teotia1 and Yogendra Singh

Abstract

In this study Box Behnken design was used to determine antimicrobial activity. The effect of independent variables (concentration of methyl and propyl paraben and pH) on the preservative efficacy was evaluated. The log reduction of different species (E.coli, S.aureus, Ps.aeruginosa, C.albicans and A.niger) at 14 day was used as response. For optimization, the effects of independent variables upon the responses were modelled using the polynomial equations involving main effects and their interactions for various responses. A graphical optimization technique was carried out to achieve optimized preservative system. The use of a design of experiment can be successful in optimisation of preservative system to meet regulatory criteria.

Keywords: Box Behnken design, log reduction, Preservative efficacy, Paraben, Optimization.