Abstract

SORPTION OF ALIZARIN RED S DYE BY CHITOSAN IN MEMBRANE AND BULK FORM

Sneha Mule* and Dhanashree Kore Dnyaneshwar Pawar

Abstract

Chitin and chitison are naturally abundant and renewable polymers have excellent properties such as biodegradability, biocompatibility, and non-toxicity. Chitosan itself chelates metal ion especially those transition metal. It can be used for immobilization of enzymes. In the present work, chitosan is immobilized in Alizarin Red-S dye. Methods: Take one gram of chitosan powder in conical flask. the homogenization of mixture in ultra Sonicator for 8- 10 hours. Keep the Petri plate along with solution in the oven for 24 hour at 500C.Due to heating treatment in the oven the transparent film formed in the Petriplate. After 24 hours the chitosan film was removed from the Petri plate. Wash with distilled water 2-3 times. Result: Immobilization of alizarin red S dye in natural chitosan in bulk form and film form Alizarin Red S dyes shows absorption maxima at 530nm. This absorbance was measured using colorimeter and was used to study the immobilization of the dye in the film.. Immobilized chitosan film is used for immobilization of Fe (II) with Alizarin Red-S dye. To check the intake capacity of chitosan film UV-Spectrophotometer is used.

Keywords: Chitin, chitison, Alizarin Red-S dye, UV-Spectrophotometer.