Abstract

SUBACUTE TOXICITY STUDIES OF METHANOLIC FRUIT EXTRACT OF VITIS VINIFERA L. IN ALBINO RATS

Muhammed Sabeeh* and Shamina S.

Abstract

In the present study, phytochemical screening was conducted to find out the secondary metabolites present in the fruit extract. The presence of phytochemicals was well found in methanolic extract when compared to others (ethanol, petroleum ether, chloroform, water). In acute toxicity study, the oral dose upto 2000 mg/kg of fruit extract was administered to three groups in single dose and general behaviour, adverse effects and mortality were determined up to 72hr. and compared to normal group. In sub-acute toxicity study, the methanolic fruit extract of Vitis vinifera L., in male albino rats at the dose levels of 150,250,350,450 and 550 mg/b.w were given at a rate of 1.0ml/rat/day to different sets of animals for 90 days. The result showed that there were no significant signs of toxicity at any dose level used in the study. Physical, biochemical as well as haematological parameters was unaltered throughout the study.

Keywords: Vitis vinifera L., methanolic extract.