Abstract

ISOLATION & CHARACTERIZATION OF MICROORGANISM FROM AGRICULTURAL SOIL FOR IDENTIFYING POTENTIAL BIOFERTILIZER FOR DRY HARSH CLIMATE OF KACHCHH, GUJARAT, INDIA

Mrugesh Trivedi*, Madhvi Joshi, Archna Kumari, Sagar Prajapati1, Karan Bhatt, Druma Vaidya1 and Neha Shukla

Abstract

The use of Chemical fertilizers has helped farmers in increasing crop yields since the 1930's. Though chemical fertilizers have an important role in increasing plant nutrients in adverse conditions or during times when crops need more nutrients, there are several adverse effects of chemical fertilizers. Some of the harmful chemical fertilizers cause soil and water pollution, chemical burns to plants, increasing air pollution, disturbing the pH of the soil and mineral leaching from the soil. The above-mentioned problems can be avoided by increasing our reliance on biofertilizers. This paper aims to find out the suitable microorganism that can be used as potential biofertilizers which are indigenous to the harsh and arid climate of the Kachchh area of Gujarat. The Fifty-six bacterial strain and four actinomycetes strain were isolated from the soil of different fields from Kukuma village of Bhuj- Kachchh (Gujarat). From the isolated organisms some strains are plant growth-promoting and some are used in to maintain nutrient stability in soil. Some strains with the greatest effects if they are used as a biofertilizer inoculum. These strains were identified to be of the genera Pseudomonas, Chryseobacterium, Escherichia, Rhizobium, Bacillus, Stenotrophomonas and Streptococcus. 16s rRNA gene sequence was used for species-level identification. Among these, some strains produced a different type of hormone, enzyme and can be used to increase crop production significantly if developed and used as biofertilizer.

Keywords: Biofertilizers, Rhizosperic soil, Phosphate solubilizing bacteria, Nitrogenfixing bacteria.