Abstract

NEUROPROTECTIVE EFFECTS OF CASSIA SIAMIA LEAVES EXTRACT IN CEREBRAL ISCHEMIA REPERFUSION INJURY INDUCED RATS

*Sravanthi K., Prasanth D., Raja B. S. and Padmasri B.

Abstract

Cerebral ischemia occurs when a blood clot has occluded a cerebral vessel. It reduces blood flow to a specific brain region, increasing the risk of cell death to that particular area. It can be either caused by thrombosis or embolism. Hence, It results in BBB injury and brain edema and also contribute to brain injury. Thus, the protective effects of cassia siamea leaf extract (CSLE) on the structure and function of the BBB were quantified in the present study. CSLE is having many antioxidant compounds such as barakol, vitamin C, Vitamin E, carotenoids, α-tocopherol, xanthophylls, tannins, flavonoid, phenolic acids, and diverse enzyme (superoxide dismutase, catalase, and peroxidase) could be responsible for this activity. we showed that CSLE (450mg/kg) treatment markedly reduced cerebral infarct area and improved neurological function in rats with cerebral ischemia– reperfusion. We also found that CSLE alleviated BBB morphological damage and attenuated increase in BBB permeability after cerebral ischemia–reperfusion. Measurement of NO, leukocytes and neutrophils in the plasma revealed that all were significantly increased in response to ischemia and reperfusion. Measurement of circulating leukocytes, indicative of a systemic inflammatory response, revealed that NO (p < 0.001) and the total number of leukocytes (p < 0.001) and neutrophils (p < 0.004) were significantly elevated 4 hours after ischemia and reperfusion. Administration of L-NA reduced the level of NO in the plasma and did not affect the cell count for leukocytes and neutrophils.

Keywords: Cassia siamea, Cerebral ischemia, Reperfusion injury Brain injury disruption of the blood–brain barrier, resulting in leukocyte transmigration into the surrounding brain tissues.