Abstract

EVALUATION OF ANTIOXIDANT ACTIVITY AND CHEMOPROTECTIVE EFFECT OF DIVINE NONI AGAINST DOXORUBICIN-INDUCED MEMORY IMPAIRMENT IN MICE

Mohammad Ali, Mohammed Rashid, Mruthunjaya Kenganora, Asher John Mohan, Nabeel Makkawi and *Manjula Santepete Nonjundaya

Abstract

Morinda citrifolia is a medicinal plant commonly known as noni. Traditionally the whole plant is used for various illness including neurological disorders. The aim of the present study was to evaluate antioxidant potential and protective effect of noni juice and divine noni gold concentrate on doxorubicin-induced memory impairment in mice. Antioxidant activity were determined by DPPH free radical scavenging assay. Protective effect of fresh noni juice and divine noni gold concentrate were assessed against doxorubicin induced memory impairment in mice using Morris water maze task. Memory impairment was induced by administrating a single i.p. dose 20 mg/kg b.w. of doxorubicin. Noni juice and divine noni gold concentrate at the dose 0.35 ml/b.w. were administered once daily for 14 days. Estimated endogenous antioxidants, malondialdehyde, acetylcholinestrase activities and histopathology were estimated. In DPPH study IC50 value of ascorbic acid, divine noni gold concentrate and noni juice was found to be 3.19 μg/ml, 150.9 μg/ml and 155.7 μg/ml respectively. Doxorubicin caused memory impairment along with increased acetylcholinestrase, malondialdehyde and decreased antioxidants activities which were reversed by noni juice and divine noni gold concentrate adjuvant therapy. The existing result is confirmed by histopathological analysis of hippocampus. Results suggest that noni juice and divine noni gold concentrate are good source of antioxidants possesses protective effect on doxorubicin induced memory impairment.

Keywords: Antioxidant, chemotherapy, memory impairment, radical scavenger, Alzheimer.