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Abstract

ETHANOL EXTRACT OF EUPHORBIA HIRTA LINN MODIFIES THE RESISTANCE OF METHICILLIN RESISTANT ΒETA LACTAMASE POSITIVE STAPHYLOCOCCUS AUREUS - AN IN VITRO STUDY

Gayathri A. and Vijaya Ramesh K.*

Abstract

The synergistic effect of Euphorbia hirta L. (inflorescence) ethanol extract (EHE) and antibiotics on drug resistant bacteria were investigated by determining the fractional inhibitory concentration (FIC) of the extract and antibiotics individually and in combination. Gram positive (β-lactamase positive Staphylococcus aureus- MRSA) bacteria was included in the study. Micro-titre plate dilution assay (96 well) was performed to calculate the MIC and FIC of the extract and for study of resistance reversal in the selected bacteria. Bio autography, a scientific method which combines chromatography with bioassay was attempted to determine the bioactive components in the ethanol extract of the inflorescence of Euphorbia hirta Linn. and Rf value calculated for the active compound.

Keywords: Euphorbia hirta L, resistance, antibiotics, MRSA, TLCType equation here.


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