Abstract

GREEN SYNTHEIS OF TITANIUM DIOXIDE NANOPARTICLES USING LAWSONIA INERMIS LEAF EXTRACT AND ITS ANTIBACTERIAL ACTIVITY

Lutfana Banu A., Soundhari C.* and Karkuzhali S.

Abstract

To biosynthesise titanium dioxide nanoparticles (TiO2 NPs) from the leaf extract of Lawsonia inermis and to determine its antibacterial activity. Green synthesis of titanium dioxide was carried out using the Aqueous leaf extract of L.inermis. The synthesised NP were lyophilised. The varying concentrations of the TiO2 NPs were tested for antibacterial activity by disc diffusion method and MBC against five clinical pathogen (Bacillus subtilis, Escherichia coli, Shigella sp, Salmonella sp, Proteus sp). The synthesized TiO2 NPs were characterized by UV-Vis spectroscopy (UV) and the size was determined by Scanning electron microscope (SEM).The UV absorbance range was from 350 nm- 360 nm. The morphological characterization of synthesized TiO2 NPs was analysed by SEM which showed spherical shape and clusters with an average size of 67.54mm. The maximum zone of inhibition was observed against Proteus sp. Green synthesized TiO2 NPs provides a promising approach to satisfy the requirements of large scale industrial production bearing the advantage of low cost, eco friendly and being reproducible.

Keywords: Lawsonia inermis, Green synthesis, Titanium dioxide nanoparticle.