Abstract

ROLE OF GRAPE SEED AND SKIN EXTRACT IN PREVENTION OF DOXORUBICIN INDUCED CARDIOTOXICITY IN RAT MODEL

Dr. Subinay Datta and Dr. Mrinal Pal

Abstract

Background: Doxorubicin is a potent chemotherapeutic drug The clinical usefulness of doxorubicin has been limited largely by the risk of cardiomyopathy and life-threatening heart failure. Cellular changes leading to this toxicity are suggested to be mediated through a druginduced increase oxidative stress. Grapeseed Proanthocyanidin [GSP] seeds possessing a potent antioxidant properties Aim: So, the present study was conducted to investigate possible protective effects of Grape seed and skin extract in doxorubicin-induced cardiotoxicity in rat model. Method: The male Wister rats (n = 34) were randomly selected and divided into three groups. The control group (n = 12) received distilled water [1ml/kg body weight] orally for 15 days, second group (n = 10) rats were injected intra peritoneal with a single dose of Doxorubicin (10mg/kg) in normal saline and in third group Doxorubicin (10mg/kg) was administered intraperitoneally and pretreatment with Grape seed and skin extract extract (200mg/kg body weight) before one hour DOX treatment by oral gavage for 15 consecutive days. Result: It was revealed by elevated serum cardiac biomarkers in comparison of control and associated with increasing levels of myocardial malondialdehyde [MDA] with simultaneously increase in the level of Superoxide dismutase. On daily oral administration of aqueous suspension of GSP seeds extract in the dose of (200 mg\kg ) for 15 days produced normalization in the serum levels of heart marker enzymes. Conclusion: The present study has demonstrated that Grape seed and its skin has got definite potency to ameliorate oxidative stress and Doxorubicin induced cardiotoxicity.

Keywords: Grape seed and skin extract, Doxorubicin, Cardiotoxicity, Oxidative stress, Antioxidant.