Abstract

RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RHIZOBIUM SP. ISOLATED FROM DOLICHOUS LABLAB

Dr. K. Anandhi*

Abstract

RFLP analysis is a technique used to identify patterns that occur in DNA. No two organisms have identical DNA, so this procedure can be used to identify if a sample of DNA came from a particular individual. Dolichous lablab is known for its dietary protein source, medicinal properties and symbiotic nitrogen fixation by Rhizobium present in its root nodules. Rhizobium sp. was identified by biochemical methods from root nodules of Dolichous lablab. High molecular weight range of R1, R2 and R3 lies between 4.07 to 3.05 kbp. RFLP assay with restriction endonuclease EcoRI used to distinguish among the three species. This RFLP assay provides an inexpensive and simple means of identifying the Rhizobium sp. RFLP analysis of R1, R2 and R3 confirm the genetic variation of the species. Genetic diversity can be identified within breeding populations in plants and animals.

Keywords: RFLP–Restriction Fragment Length Polymorphism, Genetic diversity, Restriction Endonuclease, Rhizobium, Dolichous lablab, SDS- Sodium Dodecyl Sulfate.