Abstract

IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM PTEROCARPUS SANTALINUS HEART WOOD USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND THEIR ANTIMICROBIAL ACTIVITY

Chandra Sekhar Challa, Saradamma Bulle, Lokesh T., Devanna Nayakanti and Varadacharyulu Nallanchakravarthula*

Abstract

This study aims at investigating the secondary metaboliotes and antimicrobial activity of Pterocarpus santalinus L. Heartwood. The sample of Pterocarpus santalinus L. Heartwood was analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) for secondary metabolites characterizations such as of flavonoids, saponins and steroidal sapogenins and others phenolic compounds. The results revealed that more peaks corresponding to major compounds. The compounds Pregnon-20- 0ne, CycloHexane, 1-ethyl- 1-methyl,2,4- bis(1-methyl,ethanyl), 4,5,7- trihydroxy isoflavone, Phytol, Caryophelleneoxide were identified in the peaks samples. The blue-colored spots were present at sample with two others colored spots (violet and yellow) were presented. It suggested that in vitro antimicrobial activity would be based on compounds. Sometime the compounds eluted at 5.0 and 10.0 min contribute to in vitro antimicrobial activity. The results revealed again the presence of the Terpinoids in Pterocarpus santalinus L., made for the characterization by HPLC and TLC probables flavonoids and presence of Terpinoids.

Keywords: Antimicrobial activity, Thin layer chromatography, high performance liquid chromatography, Pterocarpus santalinus L., Terpinoids.