Abstract

HISTOCHEMICAL STUDIES ON SKIN OF FRESHWATER FISH CHANNA STRIATUS INFECTED WITH BACTERIA AND FUNGI CAUSING EUS

*Koteshwar Rao. Podeti

Abstract

The harmful effect of the bacterial and fungal infection causes epizootic ulcerative syndrome (EUS). The present study focus on the histochemistry of skin of the freshwater edible fish Channa striatus infected with Aeromonas hydrophila, Staphylococcus aureus, Aspergillus fumigatus and Aspergillus niger. The histochemical observation revealed a marked reduction in the infected fishes. The glycogen content and total protein content of the skin cells was compared with the control fish. C. striatus control skin shows moderate presence of Glycogen (PAS) and infected skin shows strong the presence of Glycogen substances (PAS), C. striatus control skin shows pale blue presence of acid mucin (AB2.5pH) and infected skin shows light and dark blue presence of sulphated mucins (AB 2.5 pH), C. striatus control skin shows presence of mucin (AB 1.0 pH), infected skin shows presence of sulfated mucosubstances (ABl.0 pH) and control skin, displaying presence of Glycogen predominately (pink), less amount of mucin (blue) (AB2.5pH/PAS). C. striatus control skin, displaying presence of Glycogen predominately (pink), less amount of mucin (blue) (AB2.5pH/PAS) and infected skin displaying the presence of mucin predominately (AB 2.5 pH /PAS), control skin presented elastic fibers purple and collagen stained with light yellow (Aldehyde fuchsine) and infected skin shown elastin decreased (Aldehyde fuchsine). C. striatus control skin displaying presence of basic proteins (Bromophenol blue) and infected skin displaying depletion of basic proteins with (Bromophenol blue). The present investigation deals with the histochemical nature of the skin of C. striatus in order to discuss, the contrast among control and the effect of bacterial and fungal infected skin.

Keywords: Aeromonas hydrophila, Aspergillus niger, histochemistry, EUS, Aldehyde fuchsine.