Abstract

SCREENING OF BIOACTIVE COMPOUNDS AND STRUCTURAL ELUCIDATION FROM LEAVES OF MIRABILIS JALAPA L.

P. Sellameena and G. Santhi*

Abstract

The present investigation has been carried out to find the phytochemicals present in the leaves of M.jalapa. A preliminary phytochemical testing of the leaves extract where than to identify the phytoconstuents, which reveals that present of tannin, saponin, flovonoids, steroids, terepenoids, triterpenoid, carbohydrate, anthroquinone polyphenol, glycoside bioactive compound was isolated by column chromatography technique. The collected flovonoid fractions was purified by thin layer chromatography. FTIR and NMR studies were carried out to find the structure of bioactive compound 3,3‟,4‟,5,7-pentahydroxy flavones-3-rutinoside(Rutin).1H- NMR and 13C – NMR that reveals the structure of flavonoids. The compound was identified as 3,3‟,4‟,5,7- pentahydroxy flavones-3- rutinoside(Rutin) by 1H- NMR and 13C – NMR.

Keywords: Phytochemicals, Mirabilis jalapa L, FTIR and NMR studies.