Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT STUDY OF DUSHIVISHARI AGADA

Parvesh Kumar* and Ashish B. Goswami

Abstract

Dushivishari Agada is a compound Herbo-mineral preparation which is explained in context of Dushivisha. Present study has been planned to evaluate preliminary phyto-chemical and in-vitro antioxidant activity of Dushivishari Agada. Preliminary phytochemical analysis was performed to identify secondary metabolites and in vitro antioxidant activity was measured by means of the 1, 1-diphenyl-2- picrylhydrazyl (DPPH), Super oxide free radical scavenging assay and total polyphenol content in the aqueous extract of DVA. Phytochemical screening of aqueous extract of DVA showed the presence of Alkaloids, flavonoids, tannins and phenolic compound. Extract strongly scavenged DPPH radical and superoxide radical with the IC50 being 103.36 μg/ml and 137.73 μg/ml respectively. The extract exhibited 86.14 GAE mg/100 gm of total polyphenol content. Our study suggests that presence of these bioactive components in DVA, may serve as a natural source of antioxident for Ayurveda practitioners.

Keywords: Antioxidant, Agada, Dushivisha, Phytochemicals.