Abstract

NEPHROPROTECTIVE EFFECT OF LEAVES OF DALBERGIA LATIFOLIA ON GENTAMICIN-INDUCED NEPHROTOXICITY IN RATS

Nagarathana P. K. M., Naresh K.*, Rajendra M. and Varee Joseph Vinod

Abstract

Objective: The main objective of the study was to evaluate the effects of methanolic extract of leaves of “Dalbergia latifolia” on gentamicin - induced nephrotoxic rats. Methodology: For nephrotoxicity, thirty rats were evenly divided into 5 groups. Group I and II served as untreated and diseased control, respectively while group III were the treated group with standard drug vitamin-E. Group IV and V served as the test groups, which were pretreated with 250 and 500 mg/kg body weight per day of “Dalbergia latifolia”, 1hour before each dose of the nephrotoxicants. On the 11thday, blood samples for serum urea, serum uric acid, serum creatinine, total protein as well as ions like sodium, potassium and chloride and urine samples were collected for urinary creatinine, urinary uric acid, urinary urea, urinary total protein and the kidneys for histopathology were taken under inhaled diethyl ether anesthesia. Results: The extract shows significant nephroprotective activity in Gentamicin induced nephrotoxicity model as evident by a decrease in elevated serum creatinine, serum urea, total protein, serum uric acid, urinary urea, urinary creatinine, urinary uric acid, urinary total protein which was further confirmed by histopathological study as attenuation in tubular nephrosis, with no significant effect on ion levels and decrease in oxidative stress by increase in level of catalase, superoxide dismutase, glutathione peroxidase and decrease in levels of malondialdehyde. Conclusion: The results have shown that methanolic extract of Dalbergia latifolia can normalize the oxidative stress and attenuated tubular nephrosis and therefore it can be used as an effective nephroprotective agent against drug induced nephrotoxicity.

Keywords: Dalbergia latifolia, gentamicin, nephroprotective activity, anti-oxidant activity.