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Abstract

FREE RADICAL SCAVENGING POTENTIAL OF LEAVES OF DRYPETES SEPIARIA (WIGHT & ARN.) PAX & K. HOFFM.

Edison Dalmeida Daffodil, Pious Soris Tresina, Packia Lincy, Veerabahu Ramasamy Mohan

Abstract

The total phenolics and flavonoids and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of leaves of Drypetes sepiaria were determined using various antioxidant model system viz, DPPH, hydroxyl, superoxide, ABTS and reducing power. Total phenolic content was estimated by folinciocalteau method. Flavonoids were determined by Aluminium chloride method. In vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts were evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power using standard procedure. The total phenolics and flavonoids in methanol extract were found to be 1.128 g 100 g-1 and 0.98 g 100 g-1 respectively. Among the solvent tested, methanol extract of D. sepiaria leaf showed potent in vitro antioxidant activities. This investigation explored that, D. sepiaria leaves are a potential source of natural antioxidant.

Keywords: Drypetes sepaiaria, Antioxidant activity, Free radical, Methanol, DPPH, ABTS.


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